Receptor-independent infection of murine coronavirus: analysis by spinoculation.
Identifieur interne : 003D51 ( Main/Exploration ); précédent : 003D50; suivant : 003D52Receptor-independent infection of murine coronavirus: analysis by spinoculation.
Auteurs : Rie Watanabe [Japon] ; Shutoku Matsuyama ; Fumihiro TaguchiSource :
- Journal of virology [ 0022-538X ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Cellules HeLa, Cellules Vero, Centrifugation (), Cricetinae, Glycoprotéine de spicule des coronavirus, Glycoprotéines (déficit), Glycoprotéines (génétique), Glycoprotéines (physiologie), Glycoprotéines membranaires (métabolisme), Humains, Infections à coronavirus (métabolisme), Infections à coronavirus (virologie), Lignée cellulaire, Membrane cellulaire (métabolisme), Molécules d'adhérence cellulaire, Protéines de l'enveloppe virale (métabolisme), Récepteurs viraux (déficit), Récepteurs viraux (génétique), Récepteurs viraux (physiologie), Solubilité, Souris, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (pathogénicité), Virus de l'hépatite murine (physiologie).
- MESH :
- déficit : Glycoprotéines, Récepteurs viraux.
- génétique : Glycoprotéines, Récepteurs viraux, Virus de l'hépatite murine.
- métabolisme : Glycoprotéines membranaires, Infections à coronavirus, Membrane cellulaire, Protéines de l'enveloppe virale.
- pathogénicité : Virus de l'hépatite murine.
- physiologie : Glycoprotéines, Récepteurs viraux, Virus de l'hépatite murine.
- virologie : Infections à coronavirus.
- Animaux, Cellules HeLa, Cellules Vero, Centrifugation, Cricetinae, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Molécules d'adhérence cellulaire, Solubilité, Souris.
English descriptors
- KwdEn :
- Animals, Cell Adhesion Molecules, Cell Line, Cell Membrane (metabolism), Centrifugation (methods), Chlorocebus aethiops, Coronavirus Infections (metabolism), Coronavirus Infections (virology), Cricetinae, Glycoproteins (deficiency), Glycoproteins (genetics), Glycoproteins (physiology), HeLa Cells, Humans, Membrane Glycoproteins (metabolism), Mice, Murine hepatitis virus (genetics), Murine hepatitis virus (pathogenicity), Murine hepatitis virus (physiology), Receptors, Virus (deficiency), Receptors, Virus (genetics), Receptors, Virus (physiology), Solubility, Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (metabolism).
- MESH :
- chemical , deficiency : Glycoproteins, Receptors, Virus.
- chemical , genetics : Glycoproteins, Receptors, Virus.
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , physiology : Glycoproteins, Receptors, Virus.
- chemical : Cell Adhesion Molecules, Spike Glycoprotein, Coronavirus.
- genetics : Murine hepatitis virus.
- metabolism : Cell Membrane, Coronavirus Infections.
- methods : Centrifugation.
- pathogenicity : Murine hepatitis virus.
- physiology : Murine hepatitis virus.
- virology : Coronavirus Infections.
- Animals, Cell Line, Chlorocebus aethiops, Cricetinae, HeLa Cells, Humans, Mice, Solubility, Vero Cells.
Abstract
A highly neurovirulent murine coronavirus JHMV (wild-type [wt] JHMV) is known to spread from cells infected via the murine coronavirus mouse hepatitis virus receptor (MHVR) to cells without MHVR (MHVR-independent infection), whereas a mutant virus isolated from wt JHMV, srr7, spread only in an MHVR-dependent fashion. These observations were obtained by the overlay of JHMV-infected cells onto receptor-negative cells that are otherwise resistant to wt JHMV infection. MHVR-independent infection is hypothetically thought to be attributed to a naturally occurring fusion activation of the wt JHMV S protein, which did not occur in the case of srr7. Attachment of S protein on cells without MHVR during the S-protein activation process seems to be a key condition. Thus, in the present study, we tried to see whether wt JHMV virions that are attached on MHVR-negative cells are able to infect those cells. In order to make virions attach to the cell surface without MHVR, we have used spinoculation, namely, the centrifugation of cells together with inoculated virus at 3,000 rpm for 2 h. This procedure forces viruses to attach to the cell surface, as revealed by quantitative estimation of attached virions by real-time PCR and also facilitated wt JHMV infection to MHVR-negative cells, but failed to do so for srr7. Virions of both wt and srr7 attached on MHVR-negative cells by spinoculation were facilitated for infection in the presence of a soluble form of MHVR that induces conformational changes of both wt and srr7. It was further revealed that wt JHMV S1, but not srr7, was released from the cell surface when S protein was expressed on cells. These observations support the hypothesis that attachment of the virion to MHVR-negative cells is a critical step and that a unique feature of wt JHMV S1 to be released from S2 in a naturally occurring event is involved in an MHVR-independent infection.
DOI: 10.1128/JVI.80.10.4901-4908.2006
PubMed: 16641281
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002259
- to stream PubMed, to step Curation: 002259
- to stream PubMed, to step Checkpoint: 002040
- to stream Ncbi, to step Merge: 001477
- to stream Ncbi, to step Curation: 001477
- to stream Ncbi, to step Checkpoint: 001477
- to stream Main, to step Merge: 003F24
- to stream Main, to step Curation: 003D51
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Receptor-independent infection of murine coronavirus: analysis by spinoculation.</title><author><name sortKey="Watanabe, Rie" sort="Watanabe, Rie" uniqKey="Watanabe R" first="Rie" last="Watanabe">Rie Watanabe</name><affiliation wicri:level="1"><nlm:affiliation>Division of Respiratory Viral Diseases and SARS, Department of Virology III, National Institute of Infectious Diseases, Murayama, Tokyo 208-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Respiratory Viral Diseases and SARS, Department of Virology III, National Institute of Infectious Diseases, Murayama, Tokyo 208-0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name></author><author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16641281</idno><idno type="pmid">16641281</idno><idno type="doi">10.1128/JVI.80.10.4901-4908.2006</idno><idno type="wicri:Area/PubMed/Corpus">002259</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002259</idno><idno type="wicri:Area/PubMed/Curation">002259</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002259</idno><idno type="wicri:Area/PubMed/Checkpoint">002040</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002040</idno><idno type="wicri:Area/Ncbi/Merge">001477</idno><idno type="wicri:Area/Ncbi/Curation">001477</idno><idno type="wicri:Area/Ncbi/Checkpoint">001477</idno><idno type="wicri:doubleKey">0022-538X:2006:Watanabe R:receptor:independent:infection</idno><idno type="wicri:Area/Main/Merge">003F24</idno><idno type="wicri:Area/Main/Curation">003D51</idno><idno type="wicri:Area/Main/Exploration">003D51</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Receptor-independent infection of murine coronavirus: analysis by spinoculation.</title><author><name sortKey="Watanabe, Rie" sort="Watanabe, Rie" uniqKey="Watanabe R" first="Rie" last="Watanabe">Rie Watanabe</name><affiliation wicri:level="1"><nlm:affiliation>Division of Respiratory Viral Diseases and SARS, Department of Virology III, National Institute of Infectious Diseases, Murayama, Tokyo 208-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Respiratory Viral Diseases and SARS, Department of Virology III, National Institute of Infectious Diseases, Murayama, Tokyo 208-0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name></author><author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Adhesion Molecules</term><term>Cell Line</term><term>Cell Membrane (metabolism)</term><term>Centrifugation (methods)</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (metabolism)</term><term>Coronavirus Infections (virology)</term><term>Cricetinae</term><term>Glycoproteins (deficiency)</term><term>Glycoproteins (genetics)</term><term>Glycoproteins (physiology)</term><term>HeLa Cells</term><term>Humans</term><term>Membrane Glycoproteins (metabolism)</term><term>Mice</term><term>Murine hepatitis virus (genetics)</term><term>Murine hepatitis virus (pathogenicity)</term><term>Murine hepatitis virus (physiology)</term><term>Receptors, Virus (deficiency)</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (physiology)</term><term>Solubility</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>Centrifugation ()</term><term>Cricetinae</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines (déficit)</term><term>Glycoprotéines (génétique)</term><term>Glycoprotéines (physiologie)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Infections à coronavirus (métabolisme)</term><term>Infections à coronavirus (virologie)</term><term>Lignée cellulaire</term><term>Membrane cellulaire (métabolisme)</term><term>Molécules d'adhérence cellulaire</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Récepteurs viraux (déficit)</term><term>Récepteurs viraux (génétique)</term><term>Récepteurs viraux (physiologie)</term><term>Solubilité</term><term>Souris</term><term>Virus de l'hépatite murine (génétique)</term><term>Virus de l'hépatite murine (pathogénicité)</term><term>Virus de l'hépatite murine (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Glycoproteins</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glycoproteins</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Glycoproteins</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Cell Adhesion Molecules</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Glycoprotéines</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines</term><term>Récepteurs viraux</term><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Centrifugation</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Infections à coronavirus</term><term>Membrane cellulaire</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Glycoprotéines</term><term>Récepteurs viraux</term><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cricetinae</term><term>HeLa Cells</term><term>Humans</term><term>Mice</term><term>Solubility</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>Centrifugation</term><term>Cricetinae</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Lignée cellulaire</term><term>Molécules d'adhérence cellulaire</term><term>Solubilité</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A highly neurovirulent murine coronavirus JHMV (wild-type [wt] JHMV) is known to spread from cells infected via the murine coronavirus mouse hepatitis virus receptor (MHVR) to cells without MHVR (MHVR-independent infection), whereas a mutant virus isolated from wt JHMV, srr7, spread only in an MHVR-dependent fashion. These observations were obtained by the overlay of JHMV-infected cells onto receptor-negative cells that are otherwise resistant to wt JHMV infection. MHVR-independent infection is hypothetically thought to be attributed to a naturally occurring fusion activation of the wt JHMV S protein, which did not occur in the case of srr7. Attachment of S protein on cells without MHVR during the S-protein activation process seems to be a key condition. Thus, in the present study, we tried to see whether wt JHMV virions that are attached on MHVR-negative cells are able to infect those cells. In order to make virions attach to the cell surface without MHVR, we have used spinoculation, namely, the centrifugation of cells together with inoculated virus at 3,000 rpm for 2 h. This procedure forces viruses to attach to the cell surface, as revealed by quantitative estimation of attached virions by real-time PCR and also facilitated wt JHMV infection to MHVR-negative cells, but failed to do so for srr7. Virions of both wt and srr7 attached on MHVR-negative cells by spinoculation were facilitated for infection in the presence of a soluble form of MHVR that induces conformational changes of both wt and srr7. It was further revealed that wt JHMV S1, but not srr7, was released from the cell surface when S protein was expressed on cells. These observations support the hypothesis that attachment of the virion to MHVR-negative cells is a critical step and that a unique feature of wt JHMV S1 to be released from S2 in a naturally occurring event is involved in an MHVR-independent infection.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Watanabe, Rie" sort="Watanabe, Rie" uniqKey="Watanabe R" first="Rie" last="Watanabe">Rie Watanabe</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003D51 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003D51 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:16641281
|texte= Receptor-independent infection of murine coronavirus: analysis by spinoculation.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16641281" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |